Part:BBa_K5237003:Design

Cas Staple: SV40 NLS-dMbCas12a-dSpCas9-Nucleoplasmin NLS

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 8026
  Illegal PstI site found at 325
  Illegal PstI site found at 346
  Illegal PstI site found at 658
  Illegal PstI site found at 1813
  Illegal PstI site found at 3100
  Illegal PstI site found at 3375
  Illegal PstI site found at 4663
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 8026
  Illegal PstI site found at 325
  Illegal PstI site found at 346
  Illegal PstI site found at 658
  Illegal PstI site found at 1813
  Illegal PstI site found at 3100
  Illegal PstI site found at 3375
  Illegal PstI site found at 4663
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 8026
  Illegal BglII site found at 2276
  Illegal BglII site found at 2402
  Illegal BglII site found at 2909
  Illegal BglII site found at 2955
  Illegal BglII site found at 3002
  Illegal BglII site found at 3278
  Illegal BglII site found at 3362
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 8026
  Illegal PstI site found at 325
  Illegal PstI site found at 346
  Illegal PstI site found at 658
  Illegal PstI site found at 1813
  Illegal PstI site found at 3100
  Illegal PstI site found at 3375
  Illegal PstI site found at 4663
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 8026
  Illegal PstI site found at 325
  Illegal PstI site found at 346
  Illegal PstI site found at 658
  Illegal PstI site found at 1813
  Illegal PstI site found at 3100
  Illegal PstI site found at 3375
  Illegal PstI site found at 4663
  Illegal NgoMIV site found at 406
  Illegal NgoMIV site found at 838
  Illegal NgoMIV site found at 1405
  Illegal NgoMIV site found at 1483
  Illegal NgoMIV site found at 2455
  Illegal NgoMIV site found at 3356
  Illegal NgoMIV site found at 4951
  
• 1000
  COMPATIBLE WITH RFC[1000]